IPTG Solution, ready-to-use_10 x 1.5 mL

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10 x 1.5 mL

RH303972

R1171

1kg单位：盒

28-42品 牌：Thermo Scientific

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描述

Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon. It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.

Applications

• Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies
• Induction of gene expression under control of the lac promoter

Note
• For blue/white colony screening use 0.1 mM final IPTG concentration in LB (Luria Broth) media
• Store IPTG Solution at -20°C.
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