描述DNAzol® Reagent is a complete and ready-to-use organic reagent for the isolation of genomic DNA from solid and liquid samples of animal, plant, yeast, and bacterial origin. The DNAzol® Reagent procedure can be completed in typically 10-30 minutes with DNA recoveries of 70-100%.• Versatile – Permits the use of a broad spectrum of sample material • Efficient – Rapid isolation and high recovery of genomic DNA Efficient Isolation of Genomic DNA from a Variety of Sample Types The DNAzol® Reagent procedure uses a novel guanidine-detergent lysing solution that permits selective precipitation of DNA from cell lysate. 1 ml of DNAzol® Reagent can be used to isolate genomic DNA from 1-3 x 107 cells, from 0.1 ml of whole blood, or from 25-50 mg tissue. Rapid isolation and High Recovery of Genomic DNA The DNAzol® Reagent combines reliability, efficiency, and simplicity in its DNA isolation protocol. During isolation, a biological sample is lysed (or homogenized) in DNAzol® Reagent, and the genomic DNA is then precipitated from the lysate with ethanol. Following an ethanol wash, DNA may be solubilized in either water or 8 mM NaOH. The entire procedure can be completed in 10-30 min with DNA recovery of 70-100%. Isolated DNA can be Used for a Number of Downstream Applications: Genomic DNA extracted using the DNAzol® procedure is suitable for a variety of applications, including Southern blotting, cloning, PCR, restriction endonuclease digestion, and dot blot hybridization. For Research Use Only. Not intended for animal or human diagnostic or therapeutic use. |
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