十二烷基硫酸钠（试剂）_25G

General description

十二烷基硫酸钠（SDS）是一种阴离子去污剂，适合用于对蛋白进行变性。它具有一个带有带负电荷的硫酸盐长链脂肪族基团。这使得SDS成为一种两亲性去污剂。^[1]

Application

Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.

十二烷基硫酸钠已用于：
• 染色体免疫沉淀
• SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）
• 作为用于免疫印迹的放射免疫沉淀检测缓冲液的一种组分

阴离子洗涤剂

包装

1 kg in poly bottle

25, 100, 500 g in poly bottle

Analysis Note

针对用于变性聚丙烯酰胺凝胶电泳进行了测试。