Acetyl-alpha Tubulin (Lys40) Antibody (32-2700) in ICC/IF_100 μg
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100 μg
RH312811
32-2700
1kg单位：盒
35品 牌：热电/Thermofisher
0
Acetyl-alpha Tubulin (Lys40) Antibody (32-2700) in ICC/IF
Immunofluorescent analysis of Alpha-Tubulin (Acetylated) was done on 70% confluent log phase HeLa cells treated with 5mM sodium butyrate for 24 h. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Alpha-Tubulin (Acetylated) Recombinant Mouse Monoclonal Antibody (Product # 32-2700) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing Alpha-Tubulin (Acetylated) in cytoplasm and panel e is a no primary antibody control. The images were captured at 20X magnification.
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