孕二醇-3-葡萄糖醛酸 (PDG) 竞争性 ELISA 试剂盒_96 tests

关于本试剂盒

The Pregnanediol-3-Glucuronide (PDG) ELISA quantitates PDG in extracted serum, plasma, urine, dried fecal extracts or cell culture medium.

原理方法

The Pregnanediol-3-Glucuronide (PDG) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure PDG and its metabolites in urine and fecal samples, or in extracted serum and plasma. This kit is not recommended for serum, plasma, or saliva samples without extraction. The kit will quantitatively measure PDG present in diluted buffer samples and tissue culture media samples. A PDG standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A PDG-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to PDG to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound PDG-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.