CD8a Monoclonal Antibody (4SM15), eBioscience™试剂_25 ug

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25 ug

RH462384

14-0808-80

1kg单位：瓶

5-7品 牌：Invitrogen

0

产品详细信息

Description: The 4SM15 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Recent studies have shown the 4SM15 antibody recognizes and binds a non-CD8a epitope in neural tissue. The 4SM15 clone is not recommended for staining of mouse neural tissue. For additional information please contact our technical service (tech@eBioscience.com). For detection of mouse CD8a in FFPE mouse neural tissue please refer to alternate product Anti-Mouse CD8a Purified (cat. no 14-0195).

The 4SM15 monoclonal antibody is not recommended for immunohistochemistry of frozen mouse tissue. For detection of mouse CD8a using immunohistochemistry of frozen tissue please refer to alternate product Anti-Mouse CD8a Purified (cat. no. 14-0081).

Applications Reported: This 4SM15 antibody has been reported for use in flow cytometric analysis, microscopy, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This 4SM15 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. The 4SM15 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

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